Quantitative Fluorescence Microscopy
Quantitative Fluorescence Microscopy
Out of Print
This book is a complete guide to the technique of fluorescence microscopy. It describes the history, principles, and applications of quantitative fluorescence microscopy and also gives much practical information about the instrumentation required. In addition, there is a discussion of the exciting developments in confocal fluorescence microscopy, which allows the three-dimensional distribution of particular substances to be determined.
Reviews & endorsements
"This most recent book by F.W.D. Rost, one of the pioneers in the arena of quantitative fluorescence microscopy, has a more focused and organized content than other recent, multi-authored books on similar topics." Y-L Wang, Trends in Cell Biology
"...will be of great help to anybody embarking on quantitative fluorescence. It provides the reader with often neglected considerations on techniques, optics, fluorochromes, as well as instrumentation--all packaged neatly in a well organized, readable, rather slender volume." Boris Albini, Immunological Investigations
"...an important source of information in the field of fluorescence microscopy which is essential in view of the rapid development of confocal laser microscopy." Microscopial Society of Canada Bulletin
Product details
July 1991Hardback
9780521394222
250 pages
256 × 195 × 20 mm
0.71kg
57 b/w illus.
Unavailable - out of print January 2008
Table of Contents
- Preface
- Abbreviations and symbols
- 1. Principles of fluorescence microscopy
- 2. Instrumentation for microfluorometry
- 3. Microfluorometric technique
- 4. Microfluorometry: errors, standardization and data processing
- 5. Applications of microfluorometry
- 6. Principles of microspectrofluorometry
- 7. Instrumentation: microspectrofluorometers
- 8. Microspectrofluorometric technique
- 9. Microspectrofluorometry: errors, standardization and data processing
- 10. Applications of microspectrofluorometry
- 11. Photobleaching, photoactivation and quenching
- 12. Time-resolved fluorescence, phosphorescence and polarization
- 13. Quantitative enzyme studies
- 14. Flow cytometry Hans J. Tanke
- 15. Scanning, video intensification and image processing
- 16. History of quantitative fluorescence microscopy
- Appendices
- References
- Index.
