Fluorescence Microscopy
Fluorescence Microscopy
Out of Print
This new work in two volumes covers all aspects of fluorescence microscopy, including instrumentation, applications to a wide variety of fields, and the history of the technique. There is a chapter on quantitative techniques, including scanning, which is dealt with in more detail in a companion volume, Quantitative Fluorescence Microscopy.
Volume 1 deals with instrumentation and techniques for fluorescence microscopy, and includes the chapter on quantitation and scanning. Volume 2 deals with the applications of fluorescence microscopy in many fields. It includes information on fluorocarbons and on autofluorescence. An invaluable appendix provides an alphabetical list of fluorochromes, giving information concerning chemical structure, fluorescence properties, applications, and suitable filter combinations.
- Definitive reference work for all those using this important technique
- The technique has applications in biology, medicine and geology
- The companion volume Quantitative Fluorescence Microscopy was published 15 August 1991
Reviews & endorsements
"...a well-written, easy-to-read book which systematically discusses the different aspects of fluorescence microscopy." Microscopial Society of Canada Bulletin
"The strengths of this book are many: a succinct and understandable writing style, well-designed illustrations, and a constant focus on the practical aspects of flourescence microscopy....particularly valuable for a scientist setting up her own flourescence microscope or for an established researcher training undergraduate or graduate students in this technique. I recommend it." Karen Rose Cercone, Journal of Sedimentary Petrology
Product details
March 1992Hardback
9780521236416
267 pages
254 × 193 × 19 mm
0.817kg
61 b/w illus. 3 colour illus. 22 tables
Unavailable - out of print December 2006
Table of Contents
- List of plates
- Preface
- Abbreviations and symbols
- Principles of fluorescence microscopy
- Fluorescence: physics and chemistry
- The optical system of the fluorescence microscope
- Commercial fluorescence microscopes
- Setting up and using the fluorescence microscope
- Photography of fluorescence
- The interpretation of the fluorescence microscope image
- Quantitative and scanning fluorescence microscopy
- Appendices
- German - English vocabulary
- Checklist of features of fluorescence microscope
- The laboratory microscope
- Faults in the microscope and possible remedies
- Data on photographic films for fluorescence photomicrography
- Reciprocity failure
- Relative brightness of image on film in various formats
- Faults in photomicrographs and possible remedies
- References
- Index.
