A Practical Guide to Basic Laboratory Andrology
This practical, extensively illustrated handbook covers the procedures that are undertaken in andrology and ART laboratories to analyse and assess male-factor infertility, and to prepare spermatozoa for use in assisted conception therapy. The content is presented as brief, authoritative overviews of the relevant biological background for each area, plus detailed, step-by-step descriptions of the relevant analytical procedures. Each technical section includes quality control considerations and the optimum presentation of results. In addition to the comprehensive 'basic' semen analysis, incorporating careful analysis of sperm morphology, the handbook provides established techniques for the use of computer-aided sperm analysis and sperm functional assessment. The interpretation of laboratory results in the clinical context is highlighted throughout, and safe laboratory practice is emphasized. Fully revised, incorporating the new ISO TS 23162 on basic human semen analysis throughout, this is an invaluable resource to all scientists and technicians who perform diagnostic testing for male-factor infertility.
- Fully revised, including structured step-by-step instructions for basic analysis to give the reader an overview of the methods and proper implementation of techniques
- Detailed instructions for sperm morphology staining with each critical step explained to help troubleshooting errors
- Heavily illustrated in colour on morphology to enhance understanding
- References new ISO 23162:2021 Standard throughout
Product details
March 2022Paperback
9781009181631
380 pages
254 × 178 × 18 mm
0.82kg
Available
Table of Contents
- List of abbreviations:1. Introduction
- 2. Basic physiology
- 3. Basic semen examination
- 4. Extended semen analysis
- 5. Sperm DNA
- 6. Computer-aided sperm analysis
- 7. Sperm function tests
- 8. Tests of sperm-cervical mucus interaction
- 9. Sperm preparation
- 10. Sperm cryopreservation
- 11. Preparation of surgically retrieved spermatozoa
- 12. Quality management and accreditation
- 13. Risk management
- 14. Reproductive toxicology
- 15. Andrology laboratory safety
- Appendices:
- 16. Interpreting andrology laboratory results#
- 17. Equipment required for a basic andrology laboratory
- 18. Home testing
- 19. Example andrology laboratory forms
- 19. Comparison of replicate counts
- Index.