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A Practical Guide to Basic Laboratory Andrology

A Practical Guide to Basic Laboratory Andrology

A Practical Guide to Basic Laboratory Andrology

2nd Edition
David Mortimer, Oozoa Biomedical Inc., Vancouver
Lars Björndahl, Karolinska Institutet, Stockholm
Christopher L. R. Barratt, University of Dundee
José Antonio Castilla, HU Virgen de las Nieves, Granada
Roelof Menkveld, Stellenbosch University, South Africa
Ulrik Kvist, Karolinska Institutet, Stockholm
Juan G. Alvarez, Centro ANDROGEN, La Coruña
Trine B. Haugen, Oslo Metropolitan University
March 2022
Available
Paperback
9781009181631
£71.00
GBP
Paperback
USD
eBook

    This practical, extensively illustrated handbook covers the procedures that are undertaken in andrology and ART laboratories to analyse and assess male-factor infertility, and to prepare spermatozoa for use in assisted conception therapy. The content is presented as brief, authoritative overviews of the relevant biological background for each area, plus detailed, step-by-step descriptions of the relevant analytical procedures. Each technical section includes quality control considerations and the optimum presentation of results. In addition to the comprehensive 'basic' semen analysis, incorporating careful analysis of sperm morphology, the handbook provides established techniques for the use of computer-aided sperm analysis and sperm functional assessment. The interpretation of laboratory results in the clinical context is highlighted throughout, and safe laboratory practice is emphasized. Fully revised, incorporating the new ISO TS 23162 on basic human semen analysis throughout, this is an invaluable resource to all scientists and technicians who perform diagnostic testing for male-factor infertility.

    • Fully revised, including structured step-by-step instructions for basic analysis to give the reader an overview of the methods and proper implementation of techniques
    • Detailed instructions for sperm morphology staining with each critical step explained to help troubleshooting errors
    • Heavily illustrated in colour on morphology to enhance understanding
    • References new ISO 23162:2021 Standard throughout

    Product details

    March 2022
    Paperback
    9781009181631
    380 pages
    254 × 178 × 18 mm
    0.82kg
    Available

    Table of Contents

    • List of abbreviations:1. Introduction
    • 2. Basic physiology
    • 3. Basic semen examination
    • 4. Extended semen analysis
    • 5. Sperm DNA
    • 6. Computer-aided sperm analysis
    • 7. Sperm function tests
    • 8. Tests of sperm-cervical mucus interaction
    • 9. Sperm preparation
    • 10. Sperm cryopreservation
    • 11. Preparation of surgically retrieved spermatozoa
    • 12. Quality management and accreditation
    • 13. Risk management
    • 14. Reproductive toxicology
    • 15. Andrology laboratory safety
    • Appendices:
    • 16. Interpreting andrology laboratory results#
    • 17. Equipment required for a basic andrology laboratory
    • 18. Home testing
    • 19. Example andrology laboratory forms
    • 19. Comparison of replicate counts
    • Index.
      Authors
    • David Mortimer , Oozoa Biomedical Inc., Vancouver

      David Mortimer is President of Oozoa Biomedical Inc, Vancouver, Canada, and Honorary Senior Lecturer, University Dundee, Soctland, UK.

    • Lars Björndahl , Karolinska Institutet, Stockholm

      Lars BjÓ§rndahl is Head of the Andrology Laboratory, at the ANOVA Centre for Andrology, Sexual Medicine and Trans Gender Medicine, Karolinska University Hospital and Karolinska Institutet, Stockholm, Sweden.

    • Christopher L. R. Barratt , University of Dundee

      Christopher L. R. Barratt is Head of Division for Post Graduate Medicine and Professor of Reproductive Medicine, University of Dundee, Scotland.

    • José Antonio Castilla , HU Virgen de las Nieves, Granada

      José Antonio Castilla is Medical Director, CEIFER Biobanco – NextClinics; head of the Andrology and embryology Laboratory, Hospital Universitario Virgen de Las Nieves and Leader of the Research Group in Reproduction, Instituto de investigación biosanitaria ibs.GRANADA, Granada, Spain.

    • Roelof Menkveld , Stellenbosch University, South Africa

      Roelof Menkveld is Professor Emeritus, Department of Obstetrics & Gynecology, Faculty of Medicine and Health Sciences, Stellenbosch University, South Africa.

    • Ulrik Kvist , Karolinska Institutet, Stockholm

      Ulrik Kvist is senior researcher at the ANOVA Centre for Andrology, Sexual Medicine and Trans Gender Medicine, Karolinska University Hospital and Karolinska Institutet, Stockholm, Sweden.

    • Juan G. Alvarez , Centro ANDROGEN, La Coruña

      Juan G. Alvarez is Medical and Scientific Director of The Center of Male Infertility ANDROGEN in La Coruña, Spain; Medical and Scientific Director of the Sperm Bank BIOKIBANK in Vitoria, Alava, Spain; and Scientific Director of the IVF Center, Estudio Medico Navarro in Pamplona, Spain.

    • Trine B. Haugen , Oslo Metropolitan University

      Trine B. Haugen is Professor and Leader of the Research Group Male Reproductive Health at Department of Life Sciences and Health, OsloMet – Oslo Metropolitan University, Oslo, Norway.