Principles and Techniques of Electron Microscopy
Principles and Techniques of Electron Microscopy
Out of Print
Principles and Techniques of Electron Microscopy has established itself as a standard book for biological electron microscopists wishing to learn how to prepare their specimens for electron microscopic investigation. This fully revised and expanded 4th edition includes three new chapters covering such topics as immunocytochemistry and applications of microwave irradiation to microscopy. This revised and expanded 4th edition of Principles and Techniques of Electron Microscopy provides practical instructions on how to process biological specimens, as well as a detailed discussion on the principles underlying the various processes. The book presents methods in a self-explanatory form. Further, alternative procedures and points of disagreement help the reader interpret data accurately. What sets this book apart from its competition is that it not only describes techniques but also explains the principles behind the techniques; that is, chemical reactions underlying the use of various reagents for preserving and staining cellular components.
- Revised and expanded essential reference
- Three new chapters include coverage of plant tissues; applications of microwave irradiation to microscopy; and immunocytochemistry
- The first three editions have established this book as a classic in the field
Reviews & endorsements
' … this text is well on its way to becoming the classic introductory teaching book for biological TEM.' Microscopy and Microanalysis
' … essential to every laboratory planning to use 'state pf the art' EM techniques in the new millennium.' Nancy J. Lane, BSDB Newsletter
'… you should have it on your shelves.' J. Robin Harris, Micron 33
'Researchers, students, or technical staff who are skilled in the art, and beginners at least introduced to the techniques of electron microscopy and other forms of microscopy will find this book both pleasant and invaluable.' Biotechnic Histochemistry
Product details
July 2000Hardback
9780521632874
564 pages
264 × 211 × 29 mm
1.525kg
165 b/w illus. 33 tables
Unavailable - out of print December 2006
Table of Contents
- Preface
- 1. Hazards, precautions, and safe handling of reagents
- 2. Chemical fixation
- 3. Rinsing, dehydration and embedding
- 4. Sectioning
- 5. Support films
- 6. Positive staining
- 7. Negative staining
- 8. Low-temperature methods
- 9. Plant tissues
- 10. Applications of microwave irradiation to microscopy
- References
- Subject index.
